{"page":"\n\n
\n
\n
\n
\n
\n\nVideo\n
\n

\nPolyacrylamide Gel Electrophoresis: Protein Electrophoresis Basics\n

\n
\n
\nGet Free Access for 10 Days!\n
\n
\n
\n
\n
\n
×
\n\n\n
\n\nPublisher:\n\nCurated Video\n\n\n
\n
\nPolyacrylamide gel electrophoresis (PAGE) uses a gel matrix formed by polymerizing acrylamide and bis-acrylamide through free radicals generated by TEMED and APS. This exothermic polymerization forms linear chains cross-linked into a 3D...\nPolyacrylamide gel electrophoresis (PAGE) uses a gel matrix formed by polymerizing acrylamide and bis-acrylamide through free radicals generated by TEMED and APS. This exothermic polymerization forms linear chains cross-linked into a 3D matrix, though oxygen can interfere by neutralizing free radicals, so techniques like degassing, adding butanol, or using photopolymerization with riboflavin are used to ensure proper gel formation. Different PAGE types include SDS-PAGE, native PAGE, gradient PAGE, and 2D PAGE, each tailored for specific protein analysis needs.\n
\n
\n\n
\n
\n
\n
\n

Resource Details

\n
\n
\n
\n
Curator Rating
\n
\n
\n
\n
\n
\n
Educator Rating
Not yet Rated
\n
\n
\n
\n
\n
Media Length
\n
3:15
\n
\n
\n
\n
\n
Grade
9th - Higher Ed
\n
\n
\n
\n
\n
Subjects
Science
7 more...
\n
\n
\n
\n
\n
Media Type
Instructional Videos
\n
\n
\n
\n
\n
Source:
\n
\nBioMagica\n\n
\n
\n
\n
\n
\n
Date
\n
2022
\n
\n
\n
\n
\n\n
Language
English
\n
\n
\n
\n
\n
Audiences
For Teacher Use
2 more...
\n
\n
\n
\n
\n
Usage Permissions
Fine Print: Educational Use
\n
\n
\n
\n
\n
\n

Additional Materials

\n
\n\nSign in or Join Now
\" data-title=\"Get Full Access\" data-container=\"body\" rel=\"popover\" tabindex=\"0\" href=\"/subscription/new\">Video Transcript\n
\n
\n\nSign in or Join Now
\" data-title=\"Get Full Access\" data-container=\"body\" rel=\"popover\" tabindex=\"0\" href=\"/subscription/new\">Video Preview\n
\n
\n
\n

Concepts

\n
\n
oxygen, monomers, polymerization
\n
\n\n\n
\n
\n
\n
\n

Additional Tags

\n
\n
csir, net, page polyacrylamide gel electrophoresis, page, gel electroporosis, polyacrylamide gel electroporosis, separation of proteins, acrylamide gel, photo polymerization, csir net, set, csir ugc net, polyacrylamide gel, gel matrix, single bond, linear chains, free radicals, linear polymer, bis acrylamide, unpaired electron, acrylamide, video, electron, reactive, riboflavin, timid, creating, monomer, radical, formation, create, process, released, leading, problem, layer, solution, photopolymerization
\n
\n\n\n
\n
\n
\n
\n

Classroom Considerations

\n
Video is ad-free
\n
\n
\n

Educator Ratings

\n
\n
\n
\n
\n
\n\nShow resource details\n
\n
\n\" data-title=\"Get Full Access\" data-container=\"body\" rel=\"popover\" tabindex=\"0\" aria-label=\"Play video: Polyacrylamide Gel Electrophoresis: Protein Electrophoresis Basics\" href=\"/subscription/new\">\"Polyacrylamide\n\n
\n
\n
\n\n\n\n"}