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Using the Spectrophotometer to Analyze a Mixture
General chemistry classes practice spectroscopy. They grasp the relationships between wavelength, absorbance, and solution concentration. Additionally, they gain valuable practice using laboratory equipment such as burets and pipettes....
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The Visible Spectroscopy Expert Witness Problem
In a simulated crime science investigation, chemistry or physics sleuths use spectroscopy to analyze solutions. The instructional activity gives learners practice making salicylate solutions, using spectrophotometers, calculating...
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Spectrophotometer Determination of an Equilibrium Constant
Students determine an equilibrium constant using a spectrophotometer. In this equilibrium lesson plan, students calibrate a spectrophotometer with 5 solutions in order to prepare the spectrophotometer for the experiment. Students measure...
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Determination of Phosphorus Content in River Water
Divide your chemistry or environmental science class into two groups. Each group tests water samples from a river for the concentration of phosphorus using a different method. With chemists, you can use this activity as they learn to use...
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D Block Elements: Complex Ions and Oxidation States
Students investigate transition metals and their oxidation states. In this complex ion and oxidation states lesson plan, students use 6 transition metals and mix them with ligand solutions and oxidizing agents separately. They test their...
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Protein Assays: Bio Rad and Page Gels
Students conduct the Biuret Test and the Bio Rad Protein Assay. They examine the spectrophotometer and graph molecular weights and the concentrations of the proteins investigated. They prepare solutions for the Bio-Rad test and use the...
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Generate a Calibration Curve for Chlorophyll a
Students measure and graph the absorbance of light by chlorophyll at a specific wavelength versus chlorophyll concentration. They are given an unknown and determine its concentration from their calibration curve. Students become familiar...
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Operating the spectrophotometer
Working in small groups, young scholars explore the operation of spectrophotometer. Using the spectrophotometer, they investigate Beer's Law with everyday items.