Curated OER
Genetic Engineering/Biotechnology
Pupils create a paper model of a plasmid and insert a DNA molecule that contains a specific gene into the plasmid. They complete an actual transformation involving E. coli bacterium and relate the two activities to one another.
Curated OER
Producing a Strain of E. coli that Glows in the Dark
Students observe the experimental process called bacterial transformation and demonstrate phenotype changes in bacteria that have been transformed with an antibiotic-resistance gene and a metabolic marker. They create a luminescent...
Curated OER
The Structure of DNA
In this the structure of DNA worksheet, students read a passage about the structure of DNA in bacteria and then relate that information to answer the questions given.
Curated OER
Phenotypic and Genotypic Changes in Bacteria
Students investigate a rapid method to render the bacterium Escherichia coli "competent" to uptake plasmid DNA. They demonstrate the phenotypic changes associated with transformation. They perform a cell resuspension from the...
Curated OER
PHENOTYPIC AND GENOTYPIC CHANGES IN BACTERIA
Students use a rapid method to render the bacterium Escherichia coli "competent" in order to uptake plasmid DNA. They also have growth on the plain LB plates by the untransformed cells (which serve as controls). Students perform a cell...