Bacteria of the human intestinal microbiome generate hydrogen gas (H2). Hydrogen gas is used by these same bacteria for methanogenesis, a biochemical pathway which generates methane. Here, we will discuss this process.<br/>

Here we explore the catalytic mechanism of Myeloperoxidase and subsequent formation of Hypochlorous Acid and Hypothiocyanite, two reactive oxidative species (ROS). We will also see functions of Hypochlorous Acid.<br/>

As always, the steps of DNA electrophoresis depend on your specific lab and protocol. Here, we look at the general theory of separating DNA on an agarose gel by electrophoresis and what it is used for in the lab. We will also learn about...

In this video, we examine the conversion of cholesterol to all of the major steroid classes: progestogens, mineralocorticoids, glucocorticoids, androgens, and estrogens.

In this video, we discuss the mechanisms by which proteins are targeted to the nucleus (import) and moved from the nucleus to the cytoplasm (export). Collectively, nucleocytoplasmic transport is regulated by the nuclear pore complex and...

This video explains how to design primers for amplifying an entire gene of interest from start codon (ATG) to STOP codon. <br/>
-For<br/>ward Prim<br/>er
-Reverse Prime<br/>r
-Ordering Primers using IDT

Parkinson's disease is a neurodegenerative prion disease in which affected individuals (e.g., Michael J Fox [comedian]; Muhammad Ali [Heavyweight champ]) suffer cell death in the substantia nigra and tremors. In this video, I will...

In this episode streamed on Sept. 13, 2018, we talk about studying life in space. Plus, we share some genius space hacks for doing life science research on Earth and aboard the International Space Station.

In this video, I will show you how to design primers to amplify the entire gene during a routine PCR.

In this video, I show you:<br/>
1) How to search UniProt for a protein of interest given its na<br/&<br/>gt;me or code.
AND
2) How to access its XM numbe<br/>r and gene sequence from UniProt.

This video dissects the mechanism by which the Pancreatic β-cells sense blood glucose levels and secrete a proportional amount of insulin through negative feedback.<br/>

In this video, I show you how to... 1) obtain gene sequences from a sequencing company (e.g., GeneWiz) 2) Select which part of the sequence is best for analysis 3) analyze results with BLAST

In this video, I discuss the biosynthesis of the third aromatic amino acid, Tryptophan, from chorismate which occurs in the plastid (chloroplast).

As always, the steps of putting a gene insert into vector/plasmid AND nickel affinity chromatography depend on your specific lab and protocol. Here, we look at the general theory of engineering a poly-His tagged protein and Ni affinity...

Huntington's disease is a neurodegenerative prion disease in which affected individuals suffer destruction of neurons in the basal nuclei (e.g., GABAergic neurons) and rapid, involuntary movements as a result. In this video, I will...

Here, we explore the biosynthetic pathway for vitamin C (ascorbic acid) from glucuronic acid. Also, we explore inability of some organisms (e.g., humans) to perform this biosynthesis due to the GULO pseudogene.

Alzheimer's disease is a neurodegenerative prion disease in which affected individuals suffer destruction of cortical neurons due to accumulation of prions of both beta-amyloid and Tau type. In this video, I will explain the acquisition...

This video explores the biotin cycle. How is biotin attached to an enzyme? How is it removed? Is biotin recycled?<br/>

Did you ever wonder what happens to artificial sweeteners when you consume them in food? In this video, we track the fates of two of them (erythritol and sorbitol). They cause loose stool, but they can have other functions as well.

In this video, I explain the mechanism of progeria, a rare but fatal accelerated aging disease. We will explore the synthesis pathway for normal wild-type Lamin A and the mutant form, progerin, for which the disease is named.<br/>

Bruce D. Baker, Ed.D. is a Professor in the Department of Educational Theory, Policy, and Administration at the GSE, and is widely recognized as a leading scholar in the field of education finance. Serving in both academic and...

1) What is the function of the enzyme, lactase? 2) What is the cause of the flatulence and diarrhea associated with lactose intolerance?

Here, we will see how two forms of arsenic, arsenate and arsenote, disrupt energy metabolism via glycolysis and the TCA cycle, respectively.

In this video, we will explore the source of the major cause of the smell of flatulence. Intestinal sulfate-reducing bacteria reduce sulfate to hydrogen sulfide (H2S). We will see the biochemical pathway here and how it is coupled to...