Chromatography of Plant Pigments

This Chromatography of Plant Pigments lesson plan also includes:

Through a hands-on activity, an acetone-spinach solution is pre-made and learners use this solution to separate the pigments found in spinach using chromatography. The comprehensive resource includes an analysis and conclusion questions.

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CCSS: Adaptable
NGSS: Adaptable
Instructional Ideas

  • For best results with middle school learners, model the steps of the lab for the class
Classroom Considerations

  • Acetone is flammable; follow all safety procedures
  • You can find all materials at home, except for chromatography strips
  • Complete step one under procedure before class begins

  • Includes background information regarding photosynthesis and chlorophyll 
  • Contains five conclusion questions

  • None
Common Core