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PHENOTYPIC AND GENOTYPIC CHANGES IN BACTERIA

Students use a rapid method to render the bacterium Escherichia coli "competent" in order to uptake plasmid DNA. They also have growth on the plain LB plates by the untransformed cells (which serve as controls). Students perform a cell resuspension from the transformed and untransformed colonies, followed by a plasmid minipreparation on those cells, to enable them to demonstrate that only transformed cells have the plasmid present.

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Resource Details

Grade
10th - 12th
Subjects
Science
1 more...
Resource Type
Lesson Plans
Duration
14 days
Instructional Strategy
Inquiry-Based Learning
Usage Permissions
Fine Print
Included Materials
  • Project

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