The Cloning of Plasmid and Spinach DNA

Students will first extract genomic DNA from spinach and perform a restriction enzyme digestion on both the spinach DNA and the plasmid using the same restriction enzyme. They then ligate the cut plasmid DNA with the cut spinach DNA in order to create a recombinant plasmid.

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Resource Details

Grade
10th - 12th
Subjects
Science
1 more...
Resource Type
Lesson Plans
Duration
14 days
Instructional Strategy
Inquiry-Based Learning
Year
1993
Usage Permissions
Fine Print