Lesson Plan
Curated OER

Using a Controlled Experiment to Identify Two Unknown Plasmids

For Teachers 10th - 12th
Student demonstrate knowledge of recombinant DNA techniques (restriction enzyme digest, gel electrophoresis, and staining gel). They demonstrate skills needed to complete a gel electrophoresis and interpret a stained gel. Pupils solve...
Lesson Plan
Curated OER

Determining Cutting Site Locations

For Teachers 7th - 12th
Students explore DNA restriction mapping. They cut pieces of paper into lengths representing those produced when specific enzymes are used to cut a strand of DNA.
Lesson Plan
Curated OER

Science Lesson: Chocolate Flavored Cherries

For Teachers 9th - 12th
Students are able to identify start and stop sequences in DNA. They are able to model using restriction enzyme and ligase to remove sections of DNA and reattach them. Students are introduced to the process of recombinat DNA through the...
Lesson Plan
Curated OER

How do Restriction Enzymes Work?

For Teachers 8th - 10th
Students discover how restriction enzymes work, while learning the importance of restriction enzymes in studying DNA structure.
Lesson Plan
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Forensic Files: A DNA Dilemma

For Teachers 7th - 12th
Seventh-graders come in to science class to find a file on their desks detailing a crime to be solved! As a demonstration, you simulate the restriction of DNA samples and separate them by electrophoresis. From the gel, learners can...
Lesson Plan
Curated OER

Simulation of Gene Splicing

For Teachers 9th - 12th
Students use the exercise as a prelude to a "wet" lab or as a substitute for such a lab. It correlates well with colony transformation labs. This lab is recommended for students what have difficulty with the abstractions that genetic...
Lesson Plan
Curated OER

DNA Fingerprinting through a Chemistry Lens

For Teachers 6th - 12th
Students explore DNA and restriction enzymes using fragments of DNA separated by electrophoresis on an agarose gel. They analyze the results to see "whodunit".
Lesson Plan
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Innocent or Guilty: A Lab on DNA Gel Electrophoresis

For Teachers 8th - 12th
Learners use the prelab as an introduction to the importance of DNA fingerprinting- a form of identification that us being accepted by both scientific and leagl experts. They prepare a gel for electrophoresis. DNA fragments, which have...
Lesson Plan
Curated OER

Cut-off Genes

For Teachers 9th - 12th
Investigate the relationships between different deep-sea organisms by DNA sequencing. A worksheet provides instructions for DNA sequencing and space to work. They simulate gel electrophoresis by cutting out paper "DNA strands."...
Lesson Plan
Curated OER

Restriction Digest + DNA Comparison

For Teachers 9th - 12th
Students compare different DNA and run a gel electrophoresis.  In this DNA instructional activity students complete a DNA activity that allows them to better understand its structure and function. 
Lesson Plan
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DNA Recombination II

For Teachers 9th - 10th
Learners model recombination in DNA. They color the DNA sample and cut it out. Students use their "restriction enzymes" to cut the DNA into specific fragments. They separate the fragments using the "gel electrotrophoresis." Learners...
Lesson Plan
Curated OER

Gel Electrophoresis: Sort and See the DNA

For Teachers 8th - 12th
Students construct DNA fingerprints. In this genetics lesson, students watch an on-line gel electrophoresis animation, then they work in groups to use a restriction map to create a restriction enzyme and use that to arrange a DNA...
Lesson Plan
Curated OER

Determination of Phylogenetic Relationships Using Restriction Analysis

For Teachers 9th - 10th
Young scholars applied various mathematical analyses to interpret their data. By using the resulting DNA fragment patterns from several enzymes students established a possible evolutionary relationship among the mice.
Lesson Plan
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Molecular Explorations

For Teachers 9th - 12th
Students explain the processes for separating DNA from tissue and separating complex mixtures.  In this exploratory lesson students complete a lab where they extract DNA.  
Lesson Plan
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DNA Fingerprinting for High School Biology

For Teachers 9th - 10th
Students work with tools and techniques used in genetic engineering. They dissect a sample into fragments of DNA using restriction enzymes, sometimes called "molecular scissors". They sort these fragments, measure and analyze them according
Lesson Plan
Curated OER

Characterization of the Bioluminescence (Lux+) Gene E. coli

For Teachers Higher Ed
Students grow E. coli strain HB101, which contains the plasmid pUCD607 with the bioluminescence (Lux+) gene. The plasmid containing the Lux+ gene is isolated from the E. coli, then characterized by restriction analysis.
Lesson Plan
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Gel Electrophoresis

For Teachers 9th - 12th
Students conduct a variety of experiments to explore gel electrophoresis. For this biology lesson, explain how this process separate DNA and RNA. They discuss the practical applications of this method.
Lesson Plan
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COMPARISON OF FOUR-, SIX-, AND EIGHT-bp CUTS IN CALF THYMUS DNA

For Teachers 9th - 12th
Students use this laboratory exercise, restriction enzymes to recognize a four-, six-, and eight-nucleotide sequence. Assuming that the four component nucleotides (A, C, T, G) are distributed randomly within a DNA molecule, then any...
Lesson Plan
Curated OER

Comparison of Four-, Six-, And Eight-bp Cuts in Calf Thymus Dna

For Teachers 9th - 12th
Pupils investigate the use of restriction enzymes to recognize a four-, six-, and eight-nucleotide sequence. They utilize restrictive endonucleases to cut Thymus DNA and compare results by viewing the DNA fragments on Agarose Gel...
Lesson Plan
Curated OER

The Cloning of Plasmid and Spinach DNA

For Teachers 10th - 12th
Students will first extract genomic DNA from spinach and perform a restriction enzyme digestion on both the spinach DNA and the plasmid using the same restriction enzyme. They then ligate the cut plasmid DNA with the cut spinach DNA in...
Lesson Plan
Curated OER

Cloning of Plasmid and Spinach DNA

For Teachers 12th - Higher Ed
Students ligate fragments of genomic DNA from spinach into a vector plasmid. They perform a restriction enzyme digestion. Student use a ligated DNA molecule to transform E. coli cells. Students analyze their procedures and outcome of...
Lesson Plan
Curated OER

Gel Electrophoresis Simulation

For Teachers 9th - 10th
Students identify the steps in a gel electrophoresis experiment and describe the role of enzymes. They examine the relationship between fragment size and migration rate in a gel. They view DNA fragments as well.
Lesson Plan
Curated OER

Simulation of Gene Splicing

For Teachers 10th - 12th
Students simulate gene splicing by examining bacteria. They create a bacterial DNA into which they insert the human DNA (gene) that codes for growth hormone. They cut the sites of the enzyme to study how the human DNA can be joined to...
Lesson Plan
Curated OER

Genetics and Molecular Biology

For Teachers 9th - 10th
Pupils create DNA models using colored construction papers. In this biology lesson, students identify enzyme restriction sites. They model how DNA plasmids replicate when taken up by a bacterial cell.

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