Curated OER
Spectrophotometer Determination of an Equilibrium Constant
Students determine an equilibrium constant using a spectrophotometer. In this equilibrium lesson plan, students calibrate a spectrophotometer with 5 solutions in order to prepare the spectrophotometer for the experiment. Students measure...
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Using the Spectrophotometer to Analyze a Mixture
General chemistry classes practice spectroscopy. They grasp the relationships between wavelength, absorbance, and solution concentration. Additionally, they gain valuable practice using laboratory equipment such as burets and pipettes....
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The Visible Spectroscopy Expert Witness Problem
In a simulated crime science investigation, chemistry or physics sleuths use spectroscopy to analyze solutions. The lesson gives learners practice making salicylate solutions, using spectrophotometers, calculating dilution amounts,...
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Determination of Phosphorus Content in River Water
Divide your chemistry or environmental science class into two groups. Each group tests water samples from a river for the concentration of phosphorus using a different method. With chemists, you can use this activity as they learn to use...
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D Block Elements: Complex Ions and Oxidation States
Learners investigate transition metals and their oxidation states. In this complex ion and oxidation states lesson plan, students use 6 transition metals and mix them with ligand solutions and oxidizing agents separately. They test their...
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Using Spectrophotometers to Examine Photosynthetic Rates
High schoolers examine how photosynthetic rates can change by using different qualities of light. They practice using spectrophotometer and the scientific method to detect the absorbance values in light treatments.
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Protein Assays: Bio Rad and Page Gels
Students conduct the Biuret Test and the Bio Rad Protein Assay. They examine the spectrophotometer and graph molecular weights and the concentrations of the proteins investigated. They prepare solutions for the Bio-Rad test and use the...
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Operating the spectrophotometer
Working in small groups, students explore the operation of spectrophotometer. Using the spectrophotometer, they investigate Beer's Law with everyday items.
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Generate a Calibration Curve for Chlorophyll a
Students measure and graph the absorbance of light by chlorophyll at a specific wavelength versus chlorophyll concentration. They are given an unknown and determine its concentration from their calibration curve. Students become familiar...
National Nanotechnology Infrastructure Network
Silver Nanoparticle Synthesis and Spectroscopy
Certain materials do not always maintain the same physical properties when they exist in the nanoscale. Help your classes to explore this idea through an experimental lesson plan. Scholars use spectroscopy with samples of silver solution...
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The Space Cadet's Laboratory: Using Electromagnetic Energy to Study Astronomy
Students build their own spectrophotometer to study light. In this physics lesson, students explain the dual nature of light. They calculate the angle of incidence and refraction using Snell's law equation.
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Spectrophotometry Lab
Students use a machine called a spectrophotometer to determine how much light is absorbed by certain substances. Students record the absorbance and the % of light transmitted for different color solutions, graph their results, and then...
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# 21 Measuring Nitrate by Cadmium Reduction
Students design an experiment to evaluate the effects of various treatments on the nitrogen cycle in a freshwater aquarium. They are required to maintain a laboratory notebook of all work, measure the key analytes of the biosystem at...
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Iron in Foods
Young scholars determine the amount of iron in a sample of cereal. In this iron lesson plan, students use a computer colorimeter to measure the amount of light absorbed by the solutions. They create a calibration curve using standard...
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Graphing the Standard Curve
Students analyze colorimetric using a computer program. In this science lesson, students show application of standard curves by finding other areas of science where standard curve is analyzed.
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Obtain a visible spectra of chlorophyll a
Students become familiar with the concept of spectroscopy or the study of the interaction of matter with electromagnetic radiation. They prepare solutions and measure absorbance at different wavelengths. Pupils comprehend that mater and...
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Introduction to AP Chemistry
High schoolers are introduced to concepts in an AP Chemistry class. They work together to complete experiments on different materials. They discuss the results of the experiments.
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How Can We Calculate an Equilibrium Constant?
High schoolers explain how equilibrium quantities are changed by temperature, pressure or different concentration of substances. They work together to complete equilibrium equations. They identify the relationship between absorption of...
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USING YEAST AS AN ULTRAVIOLET LIGHT MEASUREMENT TOOL
Students realize the importance of organisms as standards of measurement and experimentation.The first lab is a simple survival curve that demonstrates the effects of UV light on cells. The second lab looks at repair mechanisms of the...
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Colorimetric Assay
Students review the basic lab techniques by following lab procedures. They analyze data by using raw data gathered. Students comprehend the processes occurring inside the column. They use specific chemicals to help determine the...
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Biotechnology
Students explore biotechnology through various activities. In this biology activity, students analyze its pros and cons. They study and read informative articles about biotechnology.
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Teaching Students How to Read the Protein Concentration in a Sample
Learners work together to identify proteins in different samples. They calculate the protein concentration in those samples as well. They complete discussion questions to end the lesson.
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What Repair Proteins are Expressed in C-Ferns After Exposure to Varying Amounts of UV Radiation?
Students examine the life cycle of c-ferns. They complete experiments on the organisms exposing them to differnet amount of UV radiation. They collect data and analyze their results.
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Beta-Oxidation of Unsaturated Fatty Acids
Students work in small groups to determine the amount of protein contained in an unknown sample. They conduct the experiment, plot the points of absorbency, and present their findings to the class.