Curated OER
A Model of a Bacterial Plasmid
Young scholars construct a plsmid model with the materials provided and be able to use it to define certain terms and explain other practical applications of genetic engineering. This simulation allows the students to work through the...
Curated OER
Using a Controlled Experiment to Identify Two Unknown Plasmids
Student demonstrate knowledge of recombinant DNA techniques (restriction enzyme digest, gel electrophoresis, and staining gel). They demonstrate skills needed to complete a gel electrophoresis and interpret a stained gel. Pupils solve...
Curated OER
CELL TRANSFORMATION IN TOBACCO LEAF DISKS
Students work with whole plant material and are not required to measure small quantities, yet they can see evidence of transformed plant cells (plant cells that have genes from bacterial plasmids). This is a laboratory suitable for...
Curated OER
Bacterial Transformation - Biology Teaching Thesis
High schoolers explain the following processes: bacteria transformation, genetic recombination, and antibacterial breakdown of bacteria. They follow lab protocol and use the scientific method: to make predications, and to explain an...
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Producing a Strain of E. coli that Glows in the Dark
Students observe the experimental process called bacterial transformation and demonstrate phenotype changes in bacteria that have been transformed with an antibiotic-resistance gene and a metabolic marker. They create a luminescent...
Curated OER
Simulation of Gene Splicing
Students simulate gene splicing by examining bacteria. They create a bacterial DNA into which they insert the human DNA (gene) that codes for growth hormone. They cut the sites of the enzyme to study how the human DNA can be joined to...
Curated OER
Vaccines
Learners explore and explain the role of vaccines in infectious disease, They emphasize immunological principles and viral/bacterial infection processes. Students explore the three main vaccine types: DNA vaccines ("gene gun"),...
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Bioterrorism: Development of a "Superbug"
Students compare bacteria and viruses and their roles in biotechnology and bioterrorism. They outline fundamental steps of bacterial transformation and the possible selection processes to identify transformants. They discuss...
Curated OER
Genetic Engineering/Biotechnology
Students create a paper model of a plasmid and insert a DNA molecule that contains a specific gene into the plasmid. They complete an actual transformation involving E. coli bacterium and relate the two activities to one another.
Curated OER
What Color is Your Colony?
Learners conduct an experiment to change the color of E. coli bacteria using genetic engineering. They work in small groups to add a plasmid containing a gene for red pigment to the bacteria and culture the bacteria to see if it ...
Curated OER
Cell Transformation in Tobacco Leaf Disks
Students review the basic principles of plant cell structure, tissue culture, sterile technique, and cell transformation. Next students complete a plant transformation experiment using whole plant material in order to view evidence of...
Curated OER
Transformation With A Firefly Gene
Learners understand the process of bacterial transformation through experimentation. They describe the function of two important genes of the pBestLuc plasmid. They explain how to induce competence in E. coli cells.
Curated OER
Bacterial Transformation
Students explore DNA recombination. In this biology lesson, students observe the growth of microscopic species. They predict the phenotype of the transformed colonies.
Curated OER
PHENOTYPIC AND GENOTYPIC CHANGES IN BACTERIA
Students use a rapid method to render the bacterium Escherichia coli "competent" in order to uptake plasmid DNA. They also have growth on the plain LB plates by the untransformed cells (which serve as controls). Students perform a cell...
Curated OER
Phenotypic and Genotypic Changes in Bacteria
Students investigate a rapid method to render the bacterium Escherichia coli "competent" to uptake plasmid DNA. They demonstrate the phenotypic changes associated with transformation. They perform a cell resuspension from the...
Curated OER
Genetics and Molecular Biology
Students create DNA models using colored construction papers. In this biology lesson, students identify enzyme restriction sites. They model how DNA plasmids replicate when taken up by a bacterial cell.
Nuffield Foundation
Following Gene Transfer by Conjugation in Bacteria
After the lab, you'll be able to solve this analogy: Natural selection is to vertical transfer of genes as ___ is to the horizontal transfer of genes. Young biologists conduct an experiment on E. coli bacteria to explore the process of...
Curated OER
Biology Demos
Students participate in biology demos. In this biology lesson plan, students explain how energy is released in the ATP cycle. They demonstrate DNA replication process.
Curated OER
Transcription, Translation, and the Genetics of Microbes
Students construct various virus models using Fruit loops and Alphabits to represent nucleotides and molecules. They constructs capsomeres using shoes as the capsomeres and discuss various viruses.